Inter Science Institute
ISI Home PageAbout ISIIndividual AssaysProfilesPatient FormsGI Medical CouncilRecent PublicationsRecent Publications

Histamine*

* Test available on a research basis only. Contact ISI for details.

 

Histamine, urine*

* Test available on a research basis only. Contact ISI for details.

 

18-Hydroxy Corticosterone (18-OH B) *

* Test available on a research basis only. Contact ISI for details.

 

18-HydroxyCorticosterone
(18-OH B), urine*

* Test available on a research basis only. Contact ISI for details.

 

18-Hydroxy 11-Deoxy Corticosterone (18-OH DOC) *

* Test available on a research basis only. Contact ISI for details.

 

18-Hydroxy
11-Deoxycorticosterone
(18OH DOC), urine*

* Test available on a research basis only. Contact ISI for details.

 

5-HIAA (5-Hydroxyindoleacetic acid), Plasma
(*Collected with ISI's Z-tubeTM)

Clinical Significance:
The ISI plasma 5-HIAA assay correlates well with the 24-hour urinary 5-HIAA assays. This test has been clinically validated for NETS patients who previously relied on the 24-hour urinary 5-HIAA. The plasma 5-HIAA saves time, alleviates the need to collect urine in a container for 24 hours, and provides equivalent clinical information.

Reference Range:
Up to 22 ng/mL

Procedure:
5-HIAA plasma is measured by GC-MS/MS.

Patient Preparation:
Patient should fast overnight prior to collection of specimen.

Specimen Collection:
Collect 10mL blood directly into ISI's Z-tubeTM Preservative and separate as soon as possible. Freeze plasma immediately after separation. Special Z-tubeTM Preservatives are available from ISI. Minimum specimen size is 1mL. Note: one Z-tubeTM may be shared for up to three tests. After centrifuging tube, aliqout 1mL per test in separate vials, mark with name of test and freeze.

Important Precaution:
Specimens for this assay must be collected using ISI's Z-tubeTM. Specimens must be shipped frozen; specimens are not stable refrigerated or ambient. No substitute preservatives are acceptable.

Special Specimens:
*Frozen Z-tubeTM plasma is the only acceptable sample type for this assay.

Shipping Instructions:
Ship specimens frozen in dry ice.

References:
1. Tellez MR, Mamikunian G, O'Dorisio TM et al. A single fasting plasma 5-HIAA value correlates with 24-hour urinary 5-HIAA values and other biomarkers in midgut neuroendocrine tumors (NETs). Pancreas. 2013:42(3): 405-410.
2. Cai H-L, Zhu R-H, Li H-D, et al. MultiSimplex optimization of chromatographic separation and dansyl derivatization conditions in the ultra performance liquid chromatography-tandem mass spectrometry analysis of neurotransmitters in human urine. J Chromato B 2011;879:1993-1999.
3. Gonzalez RR, Fernandez RF, Vidal JLM et al. Development and validation of an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous determination of neurotransmitters in rat brain samples. J Neuro Meth 2011;198: 187-194.
4. Stephanson N, Helander A, Beck O. Alcohol biomarker analysis: simultaneous determination of 5-hydroxytryptophol glucuronide and 5-hydroxyindoleacetic acid by direct injection of urine using ultra-performance liquid chromatographytandem mass spectrometry. J Mass Spect 2007;42: 940-949.

 

17-Hydroxy Pregnenolone*

* Test available on a research basis only. Contact ISI for details.

 

17-Hydroxy Pregnenolone, urine*

* Test available on a research basis only. Contact ISI for details.

 

17-Hydroxy Progesterone

Clinical Significance:
17-Hydroxy Progesterone is a steroid derived primarily from enzymatic metabolism of Progesterone and 17-Hydroxy Pregnenolone.  It is converted enzymatically to Androstenedione and 11-Deoxycortisol.  It is produced in both the gonads and adrenal glands.  It is excreted into the urine in conjugated and unconjugated forms of 17-Hydroxy Progesterone and as Pregnanetriol.  It is stimulated by ACTH and suppressed by Dexamethasone.  Levels of 17-Hydroxy Progesterone are greatly increased in patients with Polycystic Ovarian Disease and Congenital Adrenal Hyperplasia and show exaggerated responses to ACTH in these cases.  17-Hydroxy Progesterone is the marker steroid for determining cases of 21a-Hydroxylase Deficient Congenital Adrenal Hyperplasia.  Levels are frequently elevated in patients with idiopathic hirsutism. 

Reference Ranges:
Male:                                     25 - 200 ng/dl
Female:
  Follicular:                            15 -    70 ng/dl
  Luteal:                                  35 - 300 ng/dl

Procedure:
17-Hydroxy Progesterone is measured by radioimmunoassay following extraction of specimens.

Patient Preparation:
Patient should not be on any Corticosteroid, ACTH, Estrogen, or Gonadotropin medications, if possible, for at least 48 hours prior to collection of specimen.

Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible.  Minimum specimen size is 1 ml.

Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.

Shipping Instructions:
Ship specimens at room temperature or frozen in dry ice.

References:
1. E Carmina and RA Lobo.  Pituitary-Adrenal Responses to Corticotropin-Releasing Factor in Late Onset 21-Hydroxylase Deficiency.  Fertility and Sterility 54: 79-83, 1990.

2. SM Atherton, ND Barnes, and DB Grant.  Circadian Variation in Plasma 17-Hydroxyprogesterone in Patients with Congenital Adrenal Hyperplasia.  Archives of Disease in Children 47: 602, 1972

 

17-Hydroxy Progesterone, urine

Clinical Significance:
17-Hydroxy Progesterone is a steroid derived primarily from enzymatic metabolism of Progesterone and 17-Hydroxy Pregnenolone.  It is converted enzymatically to Androstenedione and 11-Deoxycortisol.  It is produced in both the gonads and adrenal glands.  It is excreted into the urine in conjugated and unconjugated forms of 17-Hydroxy Progesterone and as Pregnanetriol.  This assay measures the total of the conjugated and unconjugated forms.  It is stimulated by ACTH and supressed by Dexamethasone.  Levels of urine 17-Hydroxy Progesterone are greatly increased in patients with Polycystic Ovarian Disease and Congenital Adrenal Hyperplasia and show exaggerated responses to ACTH in these cases.  17-Hydroxy Progesterone is the marker steroid for determining cases of 21a-Hydroxylase Deficient Congenital Adrenal Hyperplasia.  Urine levels are frequently elevated in patients with idiopathic hirsutism. 

Reference Ranges:
Male:                          Up to 2.0 ug/24 hours
Female:                      Up to 4.5 ug/24 hours

Procedure:
Urine 17-Hydroxy Progesterone is measured by radioimmunoassay following extraction of specimens.

Patient Preparation:
Patient should not be on any Corticosteroid, ACTH, Estrogen, or Gonadotropin medications, if possible, for at least 48 hours prior to collection of specimen.

Specimen Collection:
10 ml of a 24 hour urine collection should be submitted for analysis. No special preservatives are required. Store specimen refrigerated during collection. Specimens should be frozen prior to shipping. Minimum specimen size is 5 ml.

Shipping Instructions:
Ship specimens frozen in dry ice. Provide the total volume per 24 hours.

References:
1. E Carmina and RA Lobo.  Pituitary-Adrenal Responses to Corticotropin-Releasing Factor in Late Onset 21-Hydroxylase Deficiency.  Fertility and Sterility 54: 79-83, 1990.

2. ET Wong, DR Brown, RA Ulstrom, and MW Steffes.  Urinary 17-alpha-Hydroxyprogesterone in Diagnosis and Management of Congenital Adrenal Hyperplasia.  Journal of Clinical Endocrinology and Metabolism 49: 377, 1979.

Please e-mail us your comments and suggestions regarding these pages. Are there other syndromes or tests that you would be interested in seeing? For our general knowledge, please tell us for which purposes you are accessing this page: general information; ordering tests; research or other(please specify). Any information gathered will be used for internal research purposes only: Confidentiality will be maintained.

 
e-mail: requests@InterScienceInstitute.com
Inter Science Institute - 944 West Hyde Park Blvd, Inglewood, CA 90302
Contact Us: Phone 800-255-2873 | 310-677-3322 | Fax: 310-677-2846