Prostaglandin D2 (PGD2) is implicated in various biological functions to mediate inflammatory
response. It is produced from arachidonic acid by the activity of two enzymes, cyclooxygenase
and tissue-specific PGD2 synthase. PGD2 has been shown to play a role in cardiovascular
diseases, arthritis, kidney fibrosis, pulmonary disease, cancer and mastocytosis. 11β-PGF2α is an
F-ring product, produced from the reduction of PGD2 by AKR1C3 and is a stereoisomer of
PGF2α. Both PGF2α and 11β-PGF2α have similar binding affinities towards the Prostaglandin F
receptor and elicit similar biological activity. 11β-PGF2α is excreted in urine and is studied in
assessing mast activation in pulmonary diseases and cancer.
< 7.1 ng/mg creatinine
Urine 11β-PGF2α is measured by direct ELISA.
Patient should not be on aspirin, indomethacin, or anti-inflammatory medications, if possible, for
at least 48 hours prior to collection of specimen.
24 hours urine collection. Refrigerate during collection and mix well. Transfer 5 – 10 ml urine to
urine transport container and freeze immediately. Rejection criteria include: room temperature,
thawed or refrigerated specimens.
Ship specimens strictly frozen in dry ice.
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