17-Hydroxy Progesterone is a steroid derived primarily from enzymatic metabolism of Progesterone and 17-Hydroxy Pregnenolone. It is converted enzymatically to Androstenedione and 11-Deoxycortisol. It is produced in both the gonads and adrenal glands. It is excreted into the urine in conjugated and unconjugated forms of 17-Hydroxy Progesterone and as Pregnanetriol. This assay measures the total of the conjugated and unconjugated forms. It is stimulated by ACTH and supressed by Dexamethasone. Levels of urine 17-Hydroxy Progesterone are greatly increased in patients with Polycystic Ovarian Disease and Congenital Adrenal Hyperplasia and show exaggerated responses to ACTH in these cases. 17-Hydroxy Progesterone is the marker steroid for determining cases of 21a-Hydroxylase Deficient Congenital Adrenal Hyperplasia. Urine levels are frequently elevated in patients with idiopathic hirsutism.
Male: Up to 2.0 ug/24 hours
Female: Up to 4.5 ug/24 hours
Urine 17-Hydroxy Progesterone is measured by radioimmunoassay.
Patient should not be on any Corticosteroid, ACTH, Estrogen, or Gonadotropin medications, if possible, for at least 48 hours prior to collection of specimen.
10 ml of a 24 hour urine collection should be submitted for analysis. No special preservatives are required. Store specimen refrigerated during collection. Specimens should be frozen prior to shipping. Minimum specimen size is 5 ml.
Ship specimens frozen in dry ice. Provide the total volume per 24 hours.
- E Carmina and RA Lobo. Pituitary-Adrenal Responses to Corticotropin-Releasing Factor in Late Onset 21-Hydroxylase Deficiency. Fertility and Sterility 54: 79-83, 1990.
- ET Wong, DR Brown, RA Ulstrom, and MW Steffes. Urinary 17-alpha-Hydroxyprogesterone in Diagnosis and Management of Congenital Adrenal Hyperplasia. Journal of Clinical Endocrinology and Metabolism 49: 377, 1979.