17-Hydroxy Progesterone is a steroid derived primarily from enzymatic metabolism of Progesterone and 17-Hydroxy Pregnenolone. It is converted enzymatically to Androstenedione and 11-Deoxycortisol. It is produced in both the gonads and adrenal glands. It is excreted into the urine in conjugated and unconjugated forms of 17-Hydroxy Progesterone and as Pregnanetriol. It is stimulated by ACTH and suppressed by Dexamethasone. Levels of 17-Hydroxy Progesterone are greatly increased in patients with Polycystic Ovarian Disease and Congenital Adrenal Hyperplasia and show exaggerated responses to ACTH in these cases. 17-Hydroxy Progesterone is the marker steroid for determining cases of 21a-Hydroxylase Deficient Congenital Adrenal Hyperplasia. Levels are frequently elevated in patients with idiopathic hirsutism.
Male: 25 – 200 ng/dl
Follicular: 15 – 70 ng/dl
Luteal: 35 – 300 ng/dl
17-Hydroxy Progesterone is measured by radioimmunoassay following extraction of specimens.
Patient should not be on any Corticosteroid, ACTH, Estrogen, or Gonadotropin medications, if possible, for at least 48 hours prior to collection of specimen.
3 ml serum or EDTA plasma should be collected and separated as soon as possible. Minimum specimen size is 1 ml.
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.
Ship specimens frozen in dry ice.
1. E Carmina and RA Lobo. Pituitary-Adrenal Responses to Corticotropin-Releasing Factor in Late Onset 21-Hydroxylase Deficiency. Fertility and Sterility 54: 79-83, 1990.
2. SM Atherton, ND Barnes, and DB Grant. Circadian Variation in Plasma 17-Hydroxyprogesterone in Patients with Congenital Adrenal Hyperplasia. Archives of Disease in Children 47: 602, 1972