Interleukin 8

Clinical Significance:
The Interleukins belong to the family termed cytokines.  They are peptides used by immune and inflammatory cells to communicate and control cell operations. The cytokines have some similar actions to the Growth Factors but Growth Factors regulate proliferation of non-immune cells.  Interleukin 8 is a 6,000 – 8000 molecular weight glycoprotein produced primarily by macrophages and monocytes.  Its primary target cells are neutrophils.  Interleukin 8 is a chemotactic agent for monocytes and neutrophils.  Interleukin 8 causes oxygen radical and enzyme release by neutrophils and mobilizes products into circulation.  Interleukin 8 is stimulated by Interleukin 1 and Tumor Necrosis Factor.

Reference Range:
Reference Range available by report.

Procedure:
Interleukin 8 is measured by direct enzyme immunoassay.

Patient Preparation:
Patient should not be on any Corticosteroids, anti-inflammatory medications or pain killers, if possible, for at least 48 hours prior to collection of specimen.

Specimen Collection:
3 ml serum or EDTA plasma should be collected and separated as soon as possible.  Freeze specimen immediately after separation.  Minimum specimen size is 1 ml.

Special Specimens:
For tumor/tissue and various fluids (i.e. CSF, peritoneal, synovial, etc.) contact the Institute for requirements and special handling.

Shipping Instructions:
Ship specimens frozen in dry ice.

References:
1. JT Whicher and SW Evans.  Cytokines in Disease.  Clinical Chemistry 36: 1269-1281, 1990.

2. K Matsushima, JJ Oppenheim.  Interleukin-8 and Macrophage Colony Stimulating Factor:  Novel Inflammatory Cytokines Inducible by Interleukin I and Tumor Necrosis Factor.  Cytokine 1: 2 – 13, 1989.